As with all assays the amounts of protein placed in the wells must be within the range where the assay result is directly proportional to protein concentration the linear range of the assay. Within the linear range of the assay 5 25 mcg ml the more protein present the more coomassie binds. The micro assay has a linear range of 15 100 µg ml using a 1 1 sample to reagent ratio.
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